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Naga figures, which include Vasuki and Takshaka, also Participate in pivotal roles in several legends, including the churning from the ocean of milk as well as the epic battles from the Mahabharata. Symbolism and Worship

For the single histidine substitutions, the N61H mutant is comparable in catalytic action on the double mutant whereas the Q59H mutant is diminished by fewer than an get of magnitude relative on the wild variety enzyme. To the substitutions at Asn-61, the replacement having an alanine is drastically a lot less disruptive compared to alter into a histidine.

Early depictions of circa-9th-century Central Java intently resembled Indic nāga which was based on imagery of cobras. In the course of this era, nāga-serpents were depicted as huge cobras supporting the waterspout of yoni-lingam.

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cat vs. pH profile, only one ionization is observed for either Zn- or Cd-NagA. This ionization need to symbolize the protonation in the metal-sure hydroxide that is utilized for substrate hydrolysis or, alternatively, the general base that is definitely used to deprotonate the metal-bound water molecule just before substrate hydrolysis. The p

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From the crystal structure with the D273N mutant of NagA, the side chain of His-143 is 3.0 Å from the phosphonate oxygen of your mimic from the tetrahedral intermediate (21). This consequence indicates this group could aid the activation of your carbonyl team of the substrate along side an conversation With all the steel ion sure throughout the active web page.

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